Detecting Rare Salamanders Using Environmental DNA

large, fat salamander with a big head and mottled olive coloration that blends in with the gravel. Its sides are wrinkled.

How do you find an elusive salamander that lives under enormous rocks at the bottom of cold, rushing streams?

The scientists at the Smithsonian Conservation Biology Institute’s Center for Species Survival (CSS) are using environmental DNA, or eDNA, to track down the hellbender, a prehistoric-looking salamander that can grow to be more than 2-feet long. Hellbenders require clean, healthy Appalachian streams but their populations are mysteriously declining in many parts of their range and we need to find efficient ways to monitor them.

With the help of more than 30 citizen scientists throughout Virginia, CSS scientists spend hours filtering gallons of stream water looking for DNA left behind by hellbenders—a kind of forensic science for wildlife conservation. In the first year of the project, the team discovered five new hellbender populations!

Continued surveillance and monitoring is needed to better understand the causes of the observed declines, and this new tool has the potential to reduce the habitat disturbance associated with physical surveillance methods.

Partners and Collaborators

  • Virginia Department of Game and Inland Fisheries
  • Virginia Master Naturalists
  • University of Virginia – Wise
  • Radford University
  • Southwest Virginia Community College
  • Cornerstone Christian Academy

Support

This work is supported by the Virginia Department of Game and Inland Fisheries, the Northeast Association of Fish and Wildlife Agencies, and the Memphis Zoo.

Continue Exploring

Changing Landscapes Initiative

Smithsonian scientists work alongside community members in Northwestern Virginia to evaluate the impacts of land-use change on wildlife, ecosystem services and community health.

Coral Biobank Alliance

Smithsonian scientists are part of the Coral Biobank Alliance, a global network of coral experts preserving corals for restoration and research.

Coral Species Cryopreserved with Global Collaborators​

View a list of the coral species that have been cryopreserved using a technique developed by Smithsonian scientists.

Wildebeest Conservation

Conservation Ecology Center scientists are tracking the movements of white-bearded wildebeest to understand how changes across the landscape impact the species.

Protecting Piping Plovers in the Great Lakes

In 2022, the Smithsonian Migratory Bird Center will begin a new research project to help protect endangered piping plovers from predation by merlins.

Swift Fox Recovery

Smithsonian scientists, in collaboration with the Fort Belknap Fish and Wildlife Department, are embarking on a five-year swift fox reintroduction project to restore swift foxes to tribal lands and to help reestablish connectivity between disjointed swift fox populations.

Conserving the World’s Largest Working Wetland

Conservation Ecology Center researchers are collaborating with institutions in Brazil and other Smithsonian colleagues to support sustainable cattle ranching in the Pantanal wetland.